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Is PAM sequence part of the sgRNA sequence construct? | IDT Genome Editing with CRISPR-Cas9 The presence of a specific protospacer adjacent motif (PAM) in the genomic DNA is required for the gRNA to bind to the target sequence. What PAM Sequence Does The PAM sequence should not be included in the design of the sgRNA. pam-site-callout (1). Cas9 nuclease, derived from S. pyogenes, recognizes a PAM sequence of gRNA design - complimentary to the target or the non target strand? ...